The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. SpectraMax Glo Steady-Luc Reporter Assay Kit Luciferase measurement for your microplate reader assay KEY FEATURES • Glow-type luminescence provides an extended signal time. 0 out of 5 $ 5,999. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. 635. Devices’ StakMax® Microplate Stacker in a matter of minutes and . It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with abs. Also refer to this Multimode Reader Comparison Chart. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. e. 72 MB. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. What is fluorescence? Fluorescence is the property of some atoms and molecules to absorb light at a particular wavelength (the excitation: Ex) followed by a short-lived emission (Em) of light at a longer wavelength (Figure 2). For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way?SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. ®The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. Wavelength Range. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. #6590-0128) • Set of 2 Fluorescence Polarization Filters 535nm BW 25nm Polarized Vertical & Horizontal (Molecular Devices cat. -36. What can be done for Communication problems with new Windows 7 computer and SoftMax Pro 5. Near bottom. 1. Recertification is recommended every two years. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 2. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). 747. Feb 22, 2023Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. For a higher degree of automation, the Automation. FLIPR Calcium 6 and 6-QF Assay Kits provide the largest signal window. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Table of Contents. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). SpectraMax Injector Cartridge with SmartInject™ Technology. These robust readers have been placed in labs from Antarctica to. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. The FlexStation 3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. The M5 has two PMTs. Softmax Pro GxP Software 6. The inset, using the same data set, highlights the sensitivity of the assay at low levels of H 2O 2. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. 100 readings per well gives optimal Z’ values for this assay. 4. Plate stacker and robot integration. The built-in micro-volume sample port allows for working with sample volumes as small as 0. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Home; Knowledge BaseThe introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. Specifications subject to change without notice. Lck kinase assay with TAMRA-labeled peptide on. D 1. The SpectraMax® M3 Multi-Mode Microplate Reader provides three modes of detection in one platform. Using a cell-based standard curve and a five-minute incubation, we observed a limit of. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86We would like to show you a description here but the site won’t allow us. measure the depth (optical pathlength) of samples with no temperature. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. Non-transfected HEK cells were included as controls. ©2022 Molecular Devices - All rights. 1) Dual Luciferase Reporter Assay (FlexStation) SpectraMax Glo Steady-Luc Reporter Assay; EarlyTox. Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax platform. Microplate Reader Shelf. With optimized reagents, option to operate the readers. Plate chamber design of the SpectraMax category 1 microplate readers. 6: bioNova científica, s. SpectraMax DuoLuc Reporter Assay (SMP 6. . view more . Bottom-reading will also give you higher RFU's (and the appearance of high sensitivity) because the polystyrene will fluoresce a bit. Call 516-939-0888 to check current. x and earlier. View the Molecular Devices websiteFigure 2. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. View the Molecular Devices website. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. It does not work as expected. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Future ready The SpectraMax i3 x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. #14-276) 。FAM标记多肽底物 (Molecular Devices Cat. 4. Molecular Devices. Molecular Devices. Web: Email: info@moldev. We would like to show you a description here but the site won’t allow us. Order Now. on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Description: Molecular Devices ’ unique SpectraDrop™ Micro-Volume Plate offers a high throughput and accurate solution for low volume measurement. Delivers single mode reader performance in one multimode reader package. For more sample throughput, the system canIntroduction. Find regional support. Plate Formats: 6, 12, 24, 48, 96, 384 Wells Plates. 01 Average RFU DNA Standard (ng. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. D. Bottom-reading is usually recommended for cell-based assay with adherent cells. Terms and Conditions Privacy Policy Online Terms of Use. ABS Plus can run both. 5. The chamber contains springs that position the cuvette in the proper alignment for a read. The following document is intended to demonstrate setup of this instrument. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Mycoplasma, the smallest and simplest of the prokaryotes, are common contaminants of cell cultures. 2. Digitizer Systems. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. Molecular Devices, LLC. 00 shippingdetection in the Molecular Devices SpectraMax M5 device. View the Molecular Devices website. Measuring cell health on the SpectraMax iD3 reader with cell viability assays. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. The chamber. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. New applications in minutes Adding modes and functionality is just minutes. Instrument. Table1-1 SpectraMax® Multi-Mode Microplate Readers and Applicable Modes Modes SpectraMax M3 SpectraMax M4 SpectraMax M5. 5. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Table of Contents. 315 can't find zero order. 1. Materials. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. SpectraMax® iD5. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. It has. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). (Molecular Devices Cat. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. #F5) Methods Standard curves were generated following the assay protocols provided with each kit. View the Molecular Devices website. If values are present, then. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. SpectraMax® ABS Plus. Both microplate and cuvette ports can be qualified. OPTICSThe trademarks used herein are the property of Molecular Devices, LLC or their respective owners. EUROPE. 1. When the user applies a set temperature of 37°C, the top is heated to 37. See SpectraMax® Microplate Reader User Manual Download Page. It has patented optimization, IQ/OQ/PM services,. SpectraMax M5 is ideal for laboratories requiring maximum flexibility in the detection of a. 2. MOLECULAR DEVICES SpectraMax M5 is an advanced multi-mode microplate reader which combines fluorescence intensity, fluorescence polarization, luminescence, absorbance and time-resolved fluorescence measurements into one integrated system. Home Resources Videos & Webinars SpectraMax M5e Multi-Mode Microplate Reader The SpectraMax M5e Multi. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. +44-118-944-8000. The donor and acceptor can be used to label a variety of biomolecules, for applications. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e. SpectraMax M5, Analyst HT or Analyst GT. To update the driver. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. In May 2011, Molecular Devices’ SpectraMax M5e became the first microplate reader in space when NanoRacks added it to their line of commercial research hardware on the International Space Station, providing researchers the ability to conduct microplate reader experiments in microgravity for the first time. Unplug the power cable from the back of the instrument. Non-transfected HEK cells were included as controls. minutes and begin reading microplates with seven mouse clicks. Published Dec 16, 2020 | Updated Dec 16, 2020 Products : Multimode Analysis. #R7159) APPLICATION NOTE 如何使用SpectraMax M5 多功能微孔. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. ©2022. -43. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Fluorescence Microplate Reader Comparison Chart. The chamber contains springs that position the cuvette in the proper alignment for a read. The following document is intended to demonstrate setup of this instrument. (“Molecular Devices”) equipment, software, reagents, and consumables to use in theWe would like to show you a description here but the site won’t allow us. 11/22 2295B. Wide range of applications; Five preferred detection modes;The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. 4. In most cases we will be able to perform the repair as listed, however in rare instances, you may be required to ship the instrument into our Service Depot Facility. SpectraMax Spectrofluorometers Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. 72 MB. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. No accessories are needed and SoftMax® Pro. 标准功. 2. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. 5. Please refer to the Obsolete Microplate Reader Models for more information. SpectraMax Plus 384 measuring instruments pdf manual download. When the user applies a set temperature of 37°C, the top is heated to 37. 2. 2. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. It has a cuvette port, spectral scanning, and up to six wavelengths per read. 2018. softmax pro software, spectramax paradigm, spectramax m5e/m5, walk-away automation, lab automation, fda 21 cfr oart 11, glp, gmp, stakmax Created Date: 1/11/2022 12:34:59. 3. This document is provided to customers who have purchased Molecular Devices, Inc. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 7°C (gradient heating design). SpectraMax Paradigm Multi-Mode Safety Guide; SpectraMax Paradigm Multi-Mode Unpacking Guide; SpectraMax Paradigm Multi-Mode. Multimode Readers Comparison Chart. Figure 3. Plot of the lower end of the DNA standard curve, ranging from 4. Oxygen consumption is one of the most informative and direct measures of mitochondrial function. Add to Cart. Spectranet customer portalMolecular Devices provides a means of validating the optical performance of the SpectraMax M2, SpectraMax M5, PLUS, PLUS 384, 190, 340PC, 340PC 384 micro-plate spectrophotometers and the VersaMax microplate reader. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Computer Cables and connectors The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. StakMax Microplate Handling System. Mary A. and California) iv SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual. Check software compatibility with Windows 10 and locate your software installer. View the Molecular Devices website. on the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. Spectranet customer portal. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). Consult Molecular Devices's entire SpectraMax M series catalogue on MedicalExpo. 7°C and the bottom to 36. 1. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Other technologies such as imaging. Molecular Devices’SpectraMax ® Microplate Readers have been validated for Transcreener ADP. Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Additional information is also available in the User Manuals. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. Select Start>Control panel>Device Manager. 5. SpectraMax M-series Microplate readers. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. View the Molecular Devices websiteすべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. To purchase a newer software version,. IP1 labeled with d2 acceptor competes with native (unlabeled) IP1 for binding to the donor-labeled IP1-specific monoclonal antibody. 5 - 2. EUROPE. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Microplate Readers include the M3, M4,. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. SoftMax Pro Software can collect data from one or more microplates orSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial numbers. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Cavicide (Warehouse, Cat # 79300360) 6. Compatible with SpectraMax iD3, iD5, i3x, i3, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, FlexStation 3 readers 0200-6191: Gray replacement validation plate case Compatible with. Also refer to this Multimode. 0A-Sunnyvale, CA-Made in USAIntroduction. HTRF-compatible SpectraMax Multi-Mode Readers. i3. SpectraMax M 系列多功能微孔板读板机包括 M2/M2e、M3、M4、M5/M5e 等几款多功能酶标仪。. Save the results of the Protocol in a data (. AMF-RF hysteretic heatingMolecular Devices microplate readers. The system provides automation for up to 50The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. ; SoftMax® Pro 7. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. . Absorbance Verification Plate (Molecular Devices, Cat # 0200-6117) 6. The Fluoroblok assays can be read on Spectramax M5/M5e, M4, M3, M2e, Gemini EM, Filtermax and Paradigm microplate readers. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi-ModeMicroplateReader SpectraMax® SpectraMaxLMicroplateReader For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way? SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The SpectraMax® M5/M5e Multimode Plate Reader is a high-performance microplate reader that can read volumes as low as 2uL and perform various detection modes for bioresearch and drug discovery applications. 40266 Email: [email protected] adapter for SoftMax Pro and SpectraMax readers. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. substrates using the SpectraMax M5 microplate reader and SoftMax® Pro Software. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Microplate Reader Shelf. SoftMax Pro Software can collect data from one or more microplates oriD5. User Manuals, Guides and Specifications for your Molecular Devices SpectraMax M5 Laboratory Equipment. Download PDF. The colorimetric signal development was stopped after 10 minutes. SpectraMax® M5 Microplate Reader Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. UV Qualification, using NIST traceable standards, is now available for SpectraMax 190, Plus 384 and the M Series (M2/M3/M4/M5/M5e) Parameters that are checked during the UV Qualification are: Wavelength accuracy. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. This integrated system provides users with a multi. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. 303 unable to cal dark current. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. Unit also passed basic testing for: Shaking, Temp, Abs, FL, Cuv Capabilities: Fluorescence (top/bottom read) Luminescence (top/bottom read) Absorbance Time-ReSpectraMax® M4. Monday to Friday 7am - 5pm Pacific Time. 1. View the Molecular Devices website. 1. Specifications subject to change without notice. l. SpectraMax M5. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. OPTICSon the SpectraMax Multi-Mode Microplate Readers by issuing a single command in SoftMax® Pro Software, Molecular Devices’ leading microplate data acquisition and analysis software platform. Published Dec 16, 2020 | Updated Dec 16, 2020. Table of Contents. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. solution for validating the performance of the SpectraMax 190 . • Software powers discovery on all Molecular Devices readers • Simplified data measurement through library of prewritten protocols. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Assay Performance with Various Instrument. Table 1. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and well-area scanning. Published Dec 16, 2020 | Updated Dec 16, 2020. 005) Photometric PrecisionScanLater Western Blot Detection Cartridge. 4. i3x. , Sigma cat. Figure 6: Transcreener ADP2 FI assay principle. 0200-0729. Molecular Devices is ISO 17025 accredited† by American Association of Laboratory Accreditation (A2LA), which requires rigorous outside auditing on a regular basis so that you can be assured of the accuracy of your certificate with respect to NIST and NMI traceable standards. peptide and phosphopeptide, read on SpectraMax M5 (quadratic curve fit). Description 1. Molecular Devices SoftMax ® Pro software was used to analyze and present the data. SpectraMax Detection Cartridge Fluorescence Polarization SpectraMax® Detection Cartridges offers great sensitivity. Download PDF. Microplate Spectrophotometers. FilterMax. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the. Optimal instrument settings are the same for all three readers. The cells were cultured in bulk flasks in DME + 10% FBS + 1% Pen/Strep/L-glutamine + 500 µg/mL of G418. SpectraMax iD3, iD5, i3x, i3, M3, M4, M5, M5e, SpectraMax L. Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!US HEADQUARTERS. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. View the Molecular Devices websiteLearn how to compare dose-response curves where there is no direct measurement of a product using Parallel line analysis - a new feature from SoftMax Pro 7 software. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Preconfigured protocols in SoftMax Pro Software expedite data. Plate Reader with Microplate Adapter, Molecular Devices SpectraMax M series Multi-mode 6. Molecular Devices SpectraMax® M5. #I2511) • Assay diluent – may be cell culture mediumThis process should take about 3 - 5 minutes. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. We would like to show you a description here but the site won’t allow us. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeMD Spectramax iD5 Plate Reader. FP assay do not perform well with protein:protein interactions. 2. Molecular DevicesSpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701). For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). UV/Vis Photometric Performance: Wavelength Range. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and. 6. book Page 1 Wednesday, July 7, 2010 2:09 PM. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch the mode to absorbance cuvette (thus the emission wavelength display disappears). Softmax Pro GxP Software 6. Check: In the data table create two new columns and add the following formulas: !Pathchecklm900. 01 0. 9. Download PDF.